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Micr0be P0rn: Powdery mildew disease of Lilac leaves

11/19/2015

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Powdery mildew organisms are highly specific for their host, and P. fraxini, the causal agent shown here, doesn't infect cannabis. We used lilac leaves and associated PM as control samples to validate some new sample preparation, staining and microscopy techniques. 

Powdery mildew genera are grouped into five tribes:
Phyllactineae, Erysipheae, Blumeriae, Golovinomyceteae, and Cystotheceae. 

The tribes can be identified by several criteria, including the type of host plant (Ex. Phyllactineae infects trees and shrubs), and by microscopic examination of their chasmothecium and appendage morphology, as well as shape and structure of spores.   


Cindy and I collected took Pee Em from a highly infected Lilac bush and brought it back to the lab. The dirty little %$#@ fungus wouldn't talk tho, and that's when things got a bit rough. We made the parasite spill its guts, all of them.

Host plant (Lilac) and appendage morphology ("spines") positively identifies genera as Phyllactinia. Organism is most probably P. fraxini, which is known to infect Lilac leaves.
Powdery mildew disease of cannabis in upcoming blog posts.
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Putting it in writing

11/19/2015

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We've been hilariously busy with various projects....and then six months flew by without a blog post. Ouch. 

Blog content will be updated weekly from this point forward, documenting some of Colorado Green Lab's projects and interest areas. 
 
Upcoming posts:
  1. Chlorophyll cleanup before flower potency testing
  2. White Ash vs Black Ash: Does this mean anything?
  3. Unintended Consequence: Development of fungicide resistance by powdery mildew disease of cannabis in Colorado 
  4. Testing: How to read a potency chromatogram
  5. Genetics: Indica and sativa strain resistance (or susceptibility) to fungal infections
​
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    Frank Conrad:

    Bio: Laboratory Director, Disambiguator, and Chief Cannabis Scientist for Colorado Green Lab.

    Cindy Blair:


    Bio: Operations Manager, Scientist Wrangler, Gatekeeper and Monitor of a very localized time/space continuum for Colorado Green Lab.
       

    Media:

    PBS: With No U.S. Standards, Pot Pesticide Use Is Rising Public Health Threat

    The Bloomberg Report: Workplace Violations Found in  Marijuana Sector.

    The Associated Press: New Threat for Pot Industry: Mildew and Pests

    The Associated Press: New marijuana industry wrestles with pesticides and safety



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